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Sunday, July 19, 2020 | History

2 edition of The zona-free hamster oocyte penetration test and the diagnosis of male fertility found in the catalog.

The zona-free hamster oocyte penetration test and the diagnosis of male fertility

The zona-free hamster oocyte penetration test and the diagnosis of male fertility

proceedings of a workshop sponsored by the World Health Organization Special programme of Research Development and Research Training in Human Reproduction, Boston, May 3, 1985

  • 206 Want to read
  • 32 Currently reading

Published by Scriptor in Copenhagen .
Written in English

    Subjects:
  • Spermatozoa -- Congresses.,
  • Fertility, Human -- Congresses.,
  • Human reproduction -- Congresses.

  • Edition Notes

    Includes bibliographies.

    Statementeditor, R.J. Aitken.
    GenreCongresses.
    SeriesInternational journal of andrology. Supplement -- no. 6., International journal of andrology -- no. 6.
    ContributionsAitken, R. J., Special Programme of Research, Development, and Research Training in Human Reproduction (World Health Organization)
    The Physical Object
    Pagination199 p. :
    Number of Pages199
    ID Numbers
    Open LibraryOL19635511M
    ISBN 108787473992

    Spermatozoa from 1 5 fertile men adhered to and penetrated 60% of hamster oocytes, whereas those from infertile men, with both normal and poor semen quality, failed to adhere or penetrate. The heterologous sperm penetration test offers a new approach to the . Tests for these steps (sperm penetration assay and IVF) are difficult to interpret as they involve 2 living cells, depend on the sequence of events and only final step can be observed. If sperm penetration test and fertilization is successful, then it is evident that at least .

    Wang C, Leung A, Tsoi W-L, Leung J, Ng V, Lee K-F, Chan SYW. Evaluation of human sperm hyperactivated motility and its relationship with the zona free hamster oocyte penetration assay. J Androl ; –8. PubMed Google Scholar.   R.J. Aitken, R.A. Elton, Application of Poisson distribution theory to the zona-free hamster oocyte penetration test to assess sperm function of men with asthenozoospermia J .

      Zona-Free Hamster Oocyte Penetration Test This test was performed as described in the WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction [ 17 ]. Immature (4- to 6-wk-old) hamster (Mesocricetus auratus) females were superovulated by an i.p. injection of 25 IU of eCG (Syntex, Buenos Aires, Argentina. Inhibition of sperm penetration of zona-free hamster eggs by CFTRinh Besides sperm capacitation and the acrosome reaction, sperm penetration of zona-free hamster eggs assay (SPA) was used to assess the human sperm fertilizing capacity, in particular the ability of sperm-oocyte fusion.


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The zona-free hamster oocyte penetration test and the diagnosis of male fertility Download PDF EPUB FB2

Get this from a library. The Zona-free hamster oocyte penetration test and the diagnosis of male fertility: proceedings of a workshop. [R J Aitken; Special Programme of Research, Development, and Research Training in Human Reproduction (World Health Organization);]. Diagnostic value of the zona-free hamster oocyte penetration test and sperm movement characteristics in oligozoospermia.

Aitken RJ. A group of 27 oligozoospermic patients were followed up for +/- years in a prospective study designed to determine which aspects of semen quality are of value in the diagnosis of by: A group of 27 oligozoospermic patients were folloved up for ± years in a prospective study designed to determine which aspects of semen quality are of value in the diagnosis of fertility.

T Cited by: Jan Kremer, Siemen Jager, The significance of the zona-free hamster oocyte test for the evaluation of male fertility**Presented at the 13th World Congress on Fertility and Sterility, Marrakesh, Morocco, October 1 to 6,Fertility and Sterility, /S(16), 54, 3, (), ().Cited by: erologous ovum penetration test, hamster zona­ free ovum test, zona-free hamster ova penetration assay, zona-free hamster oocyte test, and gamete membrane fusion test.

The word "penetration" is a misnomer because it suggests that spermatozoa actively enter the oocyte.2 The spermatozoa, how­ ever, are passively engulfed by the oocytes like par­. Barros С, Gonzalez J, Herrera E, Bustos-Obregon E () Human sperm penetration into zona-free hamster oocytes as a test to evaluate the sperm fertilizing ability.

Andrologia – PubMed CrossRef Google Scholar. The sperm–zona free hamster egg test is a heterologous bioassay that evaluates the ability of spermatozoa to bind to the oolemma and fuse with the oocyte.

We compared the expression of α6β1 on human spermatozoa by flow cytometry and ELISA with the results of the sperm-binding assay after induction of the AR to get a better insight into its. ARTICLE CITATION: Kumar S, Khan S, Sivakumar A, Yathish, HM, Sivamani B, Sharma D and Sharma A (). Zona free hamster oocyte penetration test: A reliable In Vitro bioassay to assess the bull fertility.

Adv. Anim. Vet. Sci. 1 (4): – Received: –06–30, Revised: –07–02, Accepted: –07–02 The electronic version of this article is the complete one and can be.

These tests include sperm–ZP binding assay, capacitation, hyperactivation motility, inducibility of AR, and the ability of sperm to fuse with the vitelline membrane (zona-free hamster egg penetration test). Figure 1 shows the appropriate management plan for unexplained male infertility.

Zona-free hamster oocyte penetration test Protocol Assessment of sperm chromatin PART II. SPERM PREPARATION Chapter 5 Sperm preparation techniques Introduction When spermatozoa may need to be separated from seminal plasma Choice of method The first section deals with the evaluation of the spermatozoa and includes a critical assessment of the recently introduced zona-free hamster egg test of sperm function.

Further sections include the biochemistry of gonadal function and the immunology of male reproduction; most papers are concerned with studies in the human. Aitken, R. (ed.),The zona-free hamster oocyte penetration test and the diagnosis of male fertility, Int. Androl. [Suppl.] – Google Scholar.

The outcome of this assay is hamster oocyte penetration rate expressed as the percentage of ova with positive that it is far from being an absolute test for male fertility potential. Kopf GS, Ribas J, Mastroianni L., Jr.

The zona-free hamster egg penetration assay as a prognostic indicator in a human in vitro fertilization program. Author(s): Aitken,R J; Special Programme of Research, Development, and Research Training in Human Reproduction (World Health Organization) Title(s): The Zona-free hamster oocyte penetration test and the diagnosis of male fertility: proceedings of a workshop/ sponsored by the World Health Organisation, Special Programme of Research Development and Research Training in Human.

Aitken, R.J. Diagnostic value of the zona-free hamster oocyte penetration test and sperm movement characteristics in oligozoospermia. Int. Androl., 8. II. Tests that are not consistently associated with pregnancy: Post-coital test Antisperm antibody tests Zona-free hamster egg penetration test III.

Tests that have no association with pregnancy: Endometrial biopsy Varicocele assessment Chlamydia testing Aboubakr Elnashar Laparoscopy and dye test: Indicated 1.

Abnormal HSG 2. Mid luteal progesterone for the diagnosis of ovulation ; Are useful tests, and correlate directly with the likelihood of conception. Post-coital test. Sperm penetration into cervical mucus. Hysteroscopy.

Sperm antibody tests. Varicocele assessment. Endometrial biopsy.?The sperm penetration assay in the zona-free hamster oocyte. Keywords: hamster oocyte penetration, sperm motility, unexplained infertility.

JAndrol ; Atthe heart ofthe conventional diagnosis of male infertility isadescriptive analysis ofthe semen, forwhich the principle criteria aresperm number, morphology, and motility. The assump-tion underlying this procedure isthat pregnancy. Semen culture 4.

Sperm cervical mucus interaction tests 5. Sperm function tests Computer aided sperm analysis (CASA) Sperm chromatin/DNA assays Acrosome reaction Zona free hamster oocyte penetration test Human zona pellucida binding test Sperm reactive oxygen species generation ABOUBAKR ELNASHAR 1.

In particular tests for motility and the fertilizing capacity of spermatozoa are examined, motility tests and the zona-free hamster oocyte penetration test are particularly criticized. The Authors affirm that hamster oocyte tests carried out in the presence of A have shown to correlate with fertility in vivo and may be of critical value.

Zona-free hamster oocyte test 31 2C RESEARCH TESTS 31 Sperm function tests 31 Reactive oxygen species and male infertility 31 Human zona pellucida binding tests 32 Assessment of the acrosome reaction 32 Computer-aided sperm analysis: morphology   Aitken R.J., Elton ation of a Poisson-Gamma model to study the influence of gamete concentration on sperm-oocyte fusion in the zona free hamster egg penetration test J Reprod Fertil, 78 (), p.

As a test for examining the ability of the sperm plasma membrane to fuse with the vitelline mem- brane of the oocyte, the zona-free hamster oocyte penetration test is extremely sensitive and has been shown to generate results that correlate with fertility in vivo (Aitken et al., a; Irvine and Aitken, ) and in vitro (Aitken et al., ).